Part:BBa_K2587030:Design

P_j23106_RBS_ddh_T

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 422
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 390
  Illegal NgoMIV site found at 615
  Illegal AgeI site found at 825
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We performed a side directed mutagenesis of the recognition sites of the restriction enzymes BsaI and BbsI in the coding sequence of ddh.

Source

Ddh was isolated from the genome of Corynebacterium glutamicum. The other parts were taken from the CIDAR MoClo toolbox